The former world number one posted a statement on his official website which announced that he had ended his coaching partnerships with both Stepanek and Agassi.
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The eight-time Grand Slam winner and Olympic gold medallist only began coaching Djokovic last season ahead of the French Open, ending 11 years away from the sport.
The Serb arrived in Australia with question marks over his elbow injury and has played two rounds in Melbourne wearing a compression sleeve on his serving arm.
The world No 2 split with his entire backroom team earlier in the year, following a poor start to 2017
The eight-time grand slam champion, Agassi disclosed this on Saturday